TABLE 4.
Inhibitory effects of different concentrations of PIgG on the amplification capacities of 11 thermostable DNA polymerasesa
| DNA polymerase | PCR result(s)b with the following IgG concn (μg/reaction tube):
|
|||||
|---|---|---|---|---|---|---|
| 0 | 4.1 | 0.82 | 0.41 | 0.082 | 0.041 | |
| AmpliTaq Gold | +, + | −, − | −, − | −, − | −, ± | ±, + |
| DyNAzyme II | +, + | −, −, − | ±, −, ± | ±, −, ± | +, −, ± | +, +, ± |
| DyNAzyme EXL | +, + | −, − | −, − | −, − | −, − | ±, + |
| Expand high fidelity | + | − | − | − | − | + |
| HotTub | +, + | −, − | −, − | −, − | −, − | −, − |
| Pwo | +, + | −, − | −, − | −, − | −, − | −, − |
| rTth | +, + | +, ±, ± | +, ±, ± | +, ±, ± | +, +, + | +, +, + |
| Taq | +, + | −, − | −, − | −, − | −, − | +, + |
| Tfl | +, + | ±, −, ± | ±, −, ± | ±, ±, − | −, −, − | ±, +, − |
| Tli | +, + | −, − | −, − | −, − | ±, − | +, + |
| Ultma | +, ± | −, − | −, − | −, − | −, − | −, − |
a
Concentration (wt/vol) of IgG in the PCR mixtures containing 1 ng of L. monocytogenes DNA.
b
Results of one to three independent PCRs. Symbols: +, band of high intensity; ±, band of low intensity; −, no band.