Table 1.

Mean quantification cycle (Ct), PCR efficiency, and correlation–coefficient (R²) values of miR-21 isolation from cell lines, urine, and plasma by different methods.

Method	Body Fluids				Cell Lines				Urine Sediments
	Ct	E (%)	R 2	Slope	Ct	E (%)	R2	Slope	Ct	E (%)	R 2	Slope
KCH3COOH	31.1 ± 0.4	103.54	0.995	−3.24	17.5 ± 0.07	99.5	0.992	−3.33	23.0 ± 0.3	100	0.998	−3.32
PEG4000	33.2 ± 1.0	111.5	0.993	−3.074	20.0 ± 0.13	95.49	0.996	−3.44	25.7 ± 0.45	98	1	−3.37
PEG6000	36.8 ± 0.2	91.99	0.977	−3.53	18.3 ± 0.32	116	0.983	−2.99	28.1 ± 0.74	86	0.976	−3.683
LiCl8M	34.8 ± 0.5	94.17	0.982	−3.47	21.8 ± 0.49	100.46	0.97	−3.31	31.7 ± 0.02	108	0.961	−3.145
Ethanol+LiCl	33.3 ± 0.07	99.46	0.994	−3.34	20.9 ± 0.9	105	0.998	−3.189	25.3 ± 0.62	114	0.993	−3.024
Ethanol	35.0 ± 0.09	120.02	0.979	−2.92	22.4 ± 0.03	98.03	0.991	−3.37	37.8 ± 0.63	105	0.982	−3.189

Data from three biological replicates of cell lines (HT-29 and HUVEC), body fluids (plasma), and urine samples. (Reproduced from [69]).