TABLE 2.
PGMY primer sequences
| Primer designation | Primer sequence (5′-3′) |
|---|---|
| PGMY11-A | GCA CAG GGA CAT AAC AAT GG |
| PGMY11-B | GCG CAG GGC CAC AAT AAT GG |
| PGMY11-C | GCA CAG GGA CAT AAT AAT GG |
| PGMY11-D | GCC CAG GGC CAC AAC AAT GG |
| PGMY11-E | GCT CAG GGT TTA AAC AAT GG |
| PGMY09-F | CGT CCC AAA GGA AAC TGA TC |
| PGMY09-G | CGA CCT AAA GGA AAC TGA TC |
| PGMY09-H | CGT CCA AAA GGA AAC TGA TC |
| PGMY09-Ia | G CCA AGG GGA AAC TGA TC |
| PGMY09-J | CGT CCC AAA GGA TAC TGA TC |
| PGMY09-K | CGT CCA AGG GGA TAC TGA TC |
| PGMY09-L | CGA CCT AAA GGG AAT TGA TC |
| PGMY09-M | CGA CCT AGT GGA AAT TGA TC |
| PGMY09-N | CGA CCA AGG GGA TAT TGA TC |
| PGMY09-Pa | G CCC AAC GGA AAC TGA TC |
| PGMY09-Q | CGA CCC AAG GGA AAC TGG TC |
| PGMY09-R | CGT CCT AAA GGA AAC TGG TC |
| HMB01b | GCG ACC CAA TGC AAA TTG GT |
a
PGMY09-I and PGMY09-P are 18 bp in length. The first two 5′ bases were deleted to reduce the significant internal secondary structure of the oligonucleotide.
b
HMB01 is shifted 3′ from the downstream primer region of the other HPV genotypes to avoid secondary structure formation and internal priming.