Table 2.

Regression values of responses after applying different experimental models.

Model	Y₁ (Vesicle Size, nm)
	SD	R²	Adjusted R²	Predicted R²	p-Value
Linear	12.85	0.9275	0.9108	0.8625	<0.0001
2FI	11.54	0.9551	0.9281	0.8283	0.1717
Quadratic	5.36	0.9932	0.9845	0.9328	0.0029
	Y₂ (Entrapment efficiency, %)
Linear	1.37	0.9634	0.9549	0.9242	<0.0001
2FI	0.70	0.9926	0.9881	0.9654	0.0009
Quadratic	0.38	0.9985	0.9966	0.9876	0.0077