Table 3.

ANOVA test for the quadratic model for vesicle size (Y₁) and entrapment efficiency (Y₂).

Source	Particle Size (nm)				Entrapment Efficiency (%)
	Sum of the Square	DF	F-Value	p-Value	Sum of the Square	DF	F-Value	p-Value
Model	29,427.84	9	114.00	<0.0001 *	663.11	9	521.14	<0.0001 *
A	19,700.13	1	686.83	<0.0001 *	530.89	1	3755.08	<0.0001 *
B	7727.73	1	269.42	<0.0001 *	105.42	1	745.62	<0.0001 *
C	52.69	1	1.84	0.2174 **	3.47	1	24.56	0.0016 *
AB	651.02	1	22.70	0.0020 *	3.59	1	25.40	0.0015 *
AC	162.31	1	5.66	0.0490 *	1.99	1	14.06	0.0072 *
BC	3.12	1	0.11	0.7514 **	13.80	1	97.62	<0.0001 *
A2	49.05	1	1.71	0.2323 **	0.29	1	2.08	0.1929 **
B2	206.58	1	7.20	0.0314 *	1.97	1	13.91	0.0074 *
C2	855.84	1	29.84	0.0009 *	1.88	1	13.33	0.0082 *
Residual	200.78	7		<0.0001 *	0.99	7
Lack of Fit	116.15	3	1.83	<0.0001 *	0.46	3	1.18	0.4221 **
Pure Error	84.63	4			0.52	4
Total	29,628.62	16			664.10	16

* Significant α < 0.05, ** nonsignificant α > 0.05.