FIG. 1.

Amino acid sequence alignment of the BMN1-6-related family of genes recovered from the B. microti MN1 genomic expression library screened using B. microti-infected patient sera. The relatively well conserved amino and carboxy termini flank a variable length of pattern-encoded degenerate repeats composed of six amino acids. Oligonucleotide primers BMN16-5′, BMN16-3′, BMN13-5′, BMN13-3′, and BMN112-3′ (listed in Table 1) correspond to the solid underlined amino acids. Oligonucleotide primers BMN16A-5′ and BMN16B-3′ in Table 1 relate to the dashed overlined amino acids. Boxed amino acids indicate bmn1 downstream primers RCBB6B and RCBB6D in Table 1. Boldface letters indicate amino acids which differ from the consensus sequence. In the downstream sequence alignment, dots designate termination of the clone sequence and asterisks designate consensus with the bmn1-6 sequence. The key to the shaded sequences appears at the bottom.