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. 2022 Feb 17;2022:9693064. doi: 10.1155/2022/9693064

Figure 2.

Figure 2

Functional molecule expression of DCs in patients with RSA. (a) HLA-DR, CD80, CD86, and CD274 mRNA expressions in PBMCs of NP women (n = 30) and RSA patients (n = 30) (upper panel) were detected by RT-PCR. To determine the target mRNA level, the relative intensity of the target DNA band was deduced from the ratio compared to that of a synchronous positive control β-actin (lower panel). (b) The expressions of HLA-DR, CD80, CD86, and CD274 in CD11c+ cells in PBMCs of NP women (n = 30) and RSA patients (n = 30) were analyzed by flow cytometry. Data were analyzed by unpaired Student's t-test. Mean ± SD are shown. ∗∗∗∗p < 0.0001. NP: normal pregnancy; RSA: recurrent spontaneous abortion.