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. 2022 Feb 17;2022:9693064. doi: 10.1155/2022/9693064

Table 2.

The forward and reverse primer sequences (5′-3′) of human and mouse genes used for PCR.

Primers Sequence (5′-3′)
Human HLA-DR-F AGCTGTGGACAAAGCCAACCTG
Human HLA-DR-: CTCTCAGTTCCACAGGGCTGTT
Human CD80-F AGGGGAAATGTCGCCTCTC
Human CD80-R GTCCGGTTCTTGTACTCGGG
Human CD86-F AGCACAGACACACGGATGAG
Human CD86-R AGGCCGCTTCTTCTTCTTCC
Human CD274-F TTGCTGAACGCCCCATACAA
Human CD274-R GATGAGCCCCTCAGGCATTT
Human β-actin-F AGCGAGCATCCCCCAAAGTT
Human β-actin-R GGGCACGAAGGCTCATC
Mice MHC-II-F CTGTCACGGTCGAGTGGAAA
Mice MHC-II-R CCTGTTGGCTGAAGTCCAGA
Mice CD80-F CCTCAAGTTTCCATGTCCAAGGC
Mice CD80-R GAGGAGAGTTGTAACGGCAAGG
Mice CD86-F ACGTATTGGAAGGAGATTACAGCT
Mice CD86-R TCTGTCAGCGTTACTATCCCGC
Mice CD274-F TGCGGACTACAAGCGAATCACG
Mice CD274-R TGCGGACTACAAGCGAATCACG
Mice 33D1-F ACCAGCAACCTGAACACAAGT
Mice 33D1-R ATCTTGGTTGGGCTCACCTT
Mice STAT5-F CTCCGCAGCACCAGGTAAA
Mice STAT5-R ACCTCGATGGGGAAATGCTG
Mice E2-2-F AGACACTCGCTCATGGTTGG
Mice E2-2-R TTGGCAGGAGAGAATGGCTG
Mice ID-2-F ACTCGCATCCCACTATCGTC
Mice ID-2-R GATGTCCGTGTTCAGGGTGG
Mice β-actin-F GTGACGTTGACATCCGTAAAGA
Mice β-actin-R GCCGGACTCATCGTACTCC