Fig. 6.

Kupyaphores are required for establishment of Mtb infection in vivo. (A) Study design for murine TB infection by WT and Δnrps Mtb strains. (B) Bacterial burden in the lungs of uninfected, WT and Δnrps Mtb strains infected mice as measured by CFU enumeration. (C) MRM-based LC-MS measurements of Mtb respiratory quinone, menaquinone, from the lungs of uninfected, WT, and Δnrps Mtb-infected mice. (D) Plot shows MRM-based semiquantitative LC-MS measurements of kupyaphore species: VI of m/z for [M+H]⁺ = 848.69 from lungs of uninfected, WT, and Δnrps Mtb-infected mice. Data represent mean ± SEM (n = 3 biological replicates per strain at each time point).