Table 1.
Cell or marker bead | Position (x, y), µm | Transmission image (2D), µm | SSC image (3D) | Clustering result | ||||
Surface area | Major axis | Minor axis | Perimeter | Volume, µm3 | Surface area, µm2 | |||
Marker bead #1 (T) | (0, 0) | — | — | — | — | — | — | |
Marker bead #2 (C) | (299, 98) | — | — | — | — | — | — | |
Marker bead #3 (C) | (307, 130) | — | — | — | — | — | — | |
Marker bead #4 (T) | (334, 135) | — | — | — | — | — | — | |
Marker bead #5 (C) | (541, 143) | — | — | — | — | — | — | |
Cell #1 | (887, 155) | 464.5 | 26.4 | 22.7 | 76.9 | 6,495 | 1,604 | 1 |
Cell #2 | (1,031, 149) | 648.5 | 33.2 | 24.9 | 92.3 | 10,367 | 4,532 | 1 |
Marker bead #6 (C) | (1,480, 120) | — | — | — | — | — | — | |
Marker bead #7 (C) | (1,576, 160) | — | — | — | — | — | — | |
Marker bead #8 (T) | (1,593, 172) | — | — | — | — | — | — | |
Cell #3 | (1,920, 130) | 1,125.3 | 39.2 | 36.8 | 122.1 | 23,150 | 7,705 | 2 |
Cell #4 | (2,026, 100) | 506.0 | 26.8 | 24.3 | 80.0 | 7,160 | 2,359 | 1 |
Cell #5 | (2,313, 163) | 909.7 | 34.4 | 34.2 | 109.3 | 19,712 | 4,842 | 1 |
Cell #6 | (2,379, 118) | 645.8 | 31.9 | 26.0 | 90.6 | 10,422 | 3,994 | 1 |
Marker bead #9 (T) | (2,543, 83) | — | — | — | — | — | — | |
Marker bead #10 (T) | (2,666, 37) | — | — | — | — | — | — | |
Cell #7 | (2,804, 91) | 394.0 | 23.8 | 21.7 | 74.8 | 5,327 | 2,471 | 1 |
Cell #8 | (2,890, 206) | 586.5 | 32.2 | 23.4 | 90.5 | 8,442 | 3,907 | 1 |
Cell #9 | (2,937, 217) | 814.5 | 35.0 | 29.8 | 103.7 | 15,181 | 3,705 | 1 |
Cell #10 | (3,368, 232) | 683.5 | 30.9 | 28.7 | 96.6 | 12,398 | 3,876 | 1 |
Cell #11 | (3,390, 190) | — | — | — | — | — | — | — |
Cell #12 | (3,475, 194) | 596.8 | 31.8 | 24.0 | 87.9 | 8,791 | 3,075 | 1 |
Cell #13 | (3,753, 177) | 506.0 | 28.0 | 23.2 | 81.1 | 7,210 | 2,396 | 1 |
Marker bead #1 (C) | (4,402, 187) | — | — | — | — | — | — | |
Marker bead #1 (C) | (4,431, 187) | — | — | — | — | — | — | |
Marker bead #1 (C) | (4,459, 178) | — | — | — | — | — | — |
—, not of interest.