. 2021 Dec 5;68(1):53–61. doi: 10.1262/jrd.2021-079

Table 2. In vitro development of IVF embryos derived from frozen-thawed semen prepared with GSH-supplemented semen freezing extender.

Bull	GSH concentration	Total number (No.) of oocytes cultured	No. of developed embryos (%)
Bull	GSH concentration	Total number (No.) of oocytes cultured	Cleaved	Blastocytes
1	0 mM	267	173 (64.8) ^{b A}	63 (23.6) ^A
	1 mM	245	177 (72.2) ^b	69 (28.2)
	5 mM	288	188 (65.3) ^b	84 (29.2)
	10 mM	264	212 (80.3) ^a	66 (25.0)

2	0 mM	237	45 (19.0) ^{b C}	16 (6.8) ^{b B}
	1 mM	265	86 (32.5) ^a	28 (10.6) ^ab
	5 mM	182	61 (33.5) ^a	24 (13.2) ^a
	10 mM	273	111 (40.7) ^a	42 (15.4) ^a

3	0 mM	154	114 (74.0) ^A	49 (31.8) ^A
	1 mM	ND	ND	ND
	5 mM	152	117 (77.0)	50 (32.9)
	10 mM	131	97 (71.7)	40 (30.5)

4	0 mM	240	136 (56.7) ^{b B}	63 (26.3) ^{b A}
	1 mM	ND	ND	ND
	5 mM	225	176 (78.2) ^a	93 (41.3) ^a
	10 mM	78	57 (71.7) ^a	66 (32.2) ^ab

Data from 3–9 replicates. Values with different superscripts within the same column of each bull (a–b: P < 0.05) and among bulls in the 0 mM GSH control group (A–C: P < 0.05) were significantly different. ND = not determined.