Table 4.
Summary of nucleotide exchange experiments in skinned PM fibers from DCM–D94A, HCM–D166V, and WT mouse modelsat serial pCa solutions
| DCM-D94A | WT | HCM-D166V | |||||||
| pCa | P1 (%) T1 (s) | P2 (%) T2 (s) | No. animals (F, M)No. fibers | P1 (%) T1 (s) | P2 (%) T2 (s) | No. animals (F, M) No. fibers | P1 (%) T1 (s) | P2 (%) T2 (s) | No. animals (F, M) No. fibers |
| 8 | 55 ± 3 | 42 ± 2 | 5 (3, 2)18 | 55 ± 4 | 42 ± 4 | 5 (3, 2)15 | 61 ± 3 | 35 ± 4 | 5 (4, 1)16 |
| 6.4 ± 1.6 | 138 ± 30 | 6.6 ± 1.1 | 213 ± 63 | 5.2 ± 1 | 110 ± 29 | ||||
| 7 | 55 ± 2 | 40 ± 31 | 5 (3, 2)22 | 58 ± 3 | 38 ± 3 | 5 (2, 3)15 | 62 ± 2 | 34 ± 2 | 5 (4, 1)22 |
| 5.9 ± 0.8 | 41 ± 27 | 6.8 ± 1.6 | 219 ± 50 | 5.3 ± 1 | 172 ± 44 | ||||
| 6 | 50 ± 2*,+ | 47 ± 2*, ++ | 5 (2, 3)20 | 60 ± 2 | 37 ± 2 | 5 (2, 3)15 | 61 ± 2 | 35 ± 2 | 5 (4, 1)15 |
| 6.3 ± 2.2 | 144 ± 66 | 8.3 ± 1.6 | 227 ± 46 | 4.5 ± 0.4 | 85 ± 18 | ||||
| 5 | 58 ± 3+ | 39 ± 3+ | 5 (2, 3)16 | 62 ± 4 | 35 ± 4 | 5 (2, 3)16 | 69 ± 3 | 27 ± 2 | 5 (4, 1)15 |
| 7.7 ± 1.2 | 136 ± 50 | 6.6 ± 1.7 | 207 ± 60 | 7.5 ± 0.9 | 202 ± 68 | ||||
| 4 | 56 ± 3+ | 42 ± 4+ | 5 (3, 2)20 | 60 ± 1 | 37 ± 1 | 5 (2, 3)19 | 66 ± 4 | 30 ± 4 | 5 (4, 1)15 |
| 6.8 ± 1.2 | 176 ± 63 | 9.9 ± 3.2 | 162 ± 56 | 5.1 ± 0.9 | 163 ± 56 | ||||
| Ave pCa | 55 ± 1*, ++++ | 42 ± 1*, ++++ | 6 (3, 3)96 | 59 ± 1 | 38 ± 1 | 6 (3, 3)80 | 64 ± 1* | 32 ± 1** | 5 (4, 1)83 |
| 6.6 ± 0.6 | 147 ± 21 | 7.6 ± 0.9 | 206 ± 23 | 5.6 ± 0.4 | 147 ± 21 | ||||
Data are mean ± SEM for N = no. animals. F, female; M, male. Significance was calculated by one-way (averaged pCa) or two-way ANOVA with Tukey’s multiple comparison test: *P < 0.05 and **P < 0.01 for mutant versus WT, +P < 0.05, ++P < 0.01, and ++++P < 0.0001 for DCM–D94A versus HCM–D166V.