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. 2022 Feb 25;148:112743. doi: 10.1016/j.biopha.2022.112743

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Fig. 4 — Hypothetical life cycle of SARS-CoV-2 and CRISPR-based pathway of PAC-MAN for RNA-guided degradation and suppression of viral RNA in CRISPR-Cas13-mediated suppression of coronavirus. (A) Hypothetical life cycle of SARS-CoV-2. After SARS-CoV-2 penetrates and the genome RNA is released, the positive-strand RNA genome is used as template to form genomic and sub-genomic negative-strand templates for the production of additional transcripts of viral genome and positive-stranded viral mRNAs. (B) The vial function and replication can be suppressed by Cas13d by directly targeting and cutting all viral positive-sense RNAs.