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Induction of spinal motor neurons from pMN progenitors at passage 15. (A) Schematic representation of motor neuron induction from pMN progenitors. (B) Phase-contrast images of cells at different time points of differentiation induction. (C and D) Neuron- and motor-neuron-specific gene expression after differentiation induction was analyzed by immunocytochemistry (C) and qRT‒PCR (D). MN, motor neuron. Scale bar, 100 μm. **P < 0.01.
