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. 2021 Jul 21;26(12):7582–7595. doi: 10.1038/s41380-021-01227-6

Fig. 2. Social behavior in the three-chamber test of adult male WT versus Magel2 KO and adult male WT or Magel2 KO vehicle-treated or OT-treated as neonates.

Fig. 2

A Paradigm of the three-chamber test. Sniffing time between mice is measured in each test. B WT males (N = 12) show normal behavior in all the steps of the test; Magel2 KO males (N = 9) show a significant impairment in short-term social memory. C WT mice were treated in the first week of life with vehicle or OT and then tested at 4 months. WT mice treated with vehicle (N = 18) or treated with OT (N = 10) have similar profiles with significant differences in each step of the test. D Magel2 KO mice were treated in the first week of life with vehicle or OT and then tested at 4 months. Magel2 KO-vehicle (N = 19) mice show a significant difference in the social exploration and social discrimination, but, in short-term social memory, they do not show a higher sniffing time with the novel mouse. OT-treated Magel2 KO (N = 19) mice present significant differences in each step of the test. Data represented in histograms report the interaction time (time of sniffing in seconds) as mean ± SEM. Paired t test. *P < 0.05; **P < 0.01; ***P < 0.001. Statistical analysis is reported in Supplementary Table 1.