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. 2021 Jul 30;26(12):7746–7759. doi: 10.1038/s41380-021-01238-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Experimental outline, following the protocol of Wagnon et al. [17]. B–D Experimental validation of immunization success using OVA-ELISA, GluN1_359–378-ELISA, and GluN1_168-187-ELISA. E Blood–brain-barrier (BBB) integrity assessed through Evans blue extravasation. F–I Results of behavioral phenotyping, showing locomotor activity in the open field, anxiety-related behavior in elevated plus maze, and depression-like behavior in the forced-swim test. J–L Histological quantification using 8 mice/immunization with focus on reactive gliosis, showing microglia numbers, GFAP+ area (densitometry), and representative images of quantified stainings. High-resolution images of CA1 were acquired as 10 µm Z-stacks and displayed as maximum-intensity projections. M–R Characterization of the brains’ immune cell compartment by flow cytometry of 11–12 mice/group. M Gating strategy. Quantification of CD11b^highCD45^mid cells (microglia). Quantification of CD11b^lowCD45^high leukocytes, CD4⁺ T cells, CD8⁺ T cells, and CD19⁺ B cells. Data displayed as mean ±SD.