Table 1.
Detailed presentation of the results obtained with the behavioral test battery in the DTA immunization model.
| Group 1 Control, OVA-AB | Group 2 Control, GluN1-AB | Comparison 1 vs. 2 | Group 3 DTA, OVA-AB | Group 4 DTA, GluN1-AB | Comparison 3 vs. 4 | Comparison 1 vs. 3 | Comparison 2 vs. 4 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean ± SD | n | Mean ± SD | n | test | p value | Mean ± SD | n | Mean ± SD | n | test | p value | test | p value | test | p value | |
| Health status | ||||||||||||||||
| Body weight (g): pre tamoxifen | 19.1 ± 0.8 | 16 | 19.5 ± 1.0 | 16 | t | 0.3522 | 18.9 ± 0.9 | 16 | 19.0 ± 0.9 | 14 | t | 0.6899 | t | 0.3594 | t | 0.2221 |
| Body weight (g): 10d post tamoxifen | 21.4 ± 0.8 | 16 | 21.8 ± 0.7 | 16 | t | 0.1694 | 20.8 ± 0.8 | 16 | 21.2 ± 0.9 | 14 | t | 0.2066 | t | 0.0372 | t | 0.0489 |
| Body weight (g): 79d post tamoxifen | 23.6 ± 0.8 | 16 | 23.5 ± 0.8 | 16 | t | 0.6761 | 23.2 ± 1.0 | 16 | 23.2 ± 0.9 | 14 | t | 0.9760 | t | 0.1886 | t | 0.3524 |
| Food intake (g): pre tamoxifen | 10.9 ± 1.0 | 16 | 10.5 ± 1.3 | 16 | t | 0.3044 | 10.5 ± 1.0 | 16 | 10.8 ± 1.1 | 14 | t | 0.3649 | t | 0.2004 | t | 0.4688 |
| Food intake (g): post tamoxifen | 11.7 ± 1.1 | 16 | 12.5 ± 1.3 | 16 | t | 0.0659 | 12.1 ± 1.7 | 16 | 12.1 ± 1.4 | 14 | t | 0.9875 | t | 0.4654 | t | 0.3806 |
| Water intake (g): pre tamoxifen | 13.1 ± 2.3 | 16 | 12.8 ± 1.5 | 16 | U | 0.8744 | 12.3 ± 1.8 | 16 | 13.0 ± 1.7 | 14 | t | 0.2818 | U | 0.4962 | t | 0.7581 |
| Water intake (g): post tamoxifen | 12.1 ± 1.3 | 16 | 12.3 ± 1.3 | 16 | t | 0.6492 | 12.3 ± 1.6 | 16 | 11.7 ± 1.2 | 14 | t | 0.2722 | t | 0.7220 | t | 0.1966 |
| Locomotor activity | ||||||||||||||||
| LABORAS (baseline): Time in locomotion (s) | 1798 ± 335 | 16 | 1804 ± 339 | 16 | t | 0.9598 | 1945 ± 392 | 16 | 1852 ± 712 | 14 | t | 0.6676 | t | 0.2613 | t | 0.8192 |
| LABORAS (post tam.): Time in locomotion (s) | 2092 ± 453 | 16 | 2096 ± 666 | 15 | t | 0.9836 | 3093 ± 872 | 16 | 3129 ± 909 | 14 | t | 0.9113 | t | 0.0005 | t | 0.0020 |
| Complex wheel running: Distance (m) | 1195 ± 729 | 6 | 1414 ± 556 | 6 | t | 0.5726 | 1841 ± 453 | 6 | 2045 ± 269 | 6 | t | 0.3691 | t | 0.1010 | t | 0.0400 |
| IC: Total corner visits (#) | 1420 ± 245 | 16 | 1506 ± 326 | 16 | t | 0.4071 | 1741 ± 288 | 16 | 2181 ± 321 | 14 | t | 0.0005 | t | 0.0020 | t | <0.0001 |
| IC: Corner visits in dark phase (#) | 1076 ± 177 | 16 | 1136 ± 262 | 16 | t | 0.4547 | 1400 ± 270 | 16 | 1713 ± 264 | 14 | t | 0.0033 | t | 0.0005 | t | <0.0001 |
| IC: Corner visits in light phase (#) | 344 ± 81 | 16 | 370 ± 81 | 16 | t | 0.3744 | 341 ± 85 | 16 | 468 ± 94 | 14 | t | 0.0006 | t | 0.9142 | t | 0.0052 |
| Dark vs. light phase | t-test | p < 0.0001 | t-test | p < 0.0001 | t-test | p < 0.0001 | t-test | p < 0.0001 | ||||||||
| Number of circles (#) | 774 ± 248 | 16 | 772 ± 305 | 15 | t | 0.9813 | 874 ± 393 | 16 | 849 ± 283 | 14 | U | 0.6896 | U | 0.6689 | t | 0.4874 |
| LABORAS phenotyping after tamoxifen-induction (activity) | ||||||||||||||||
| Time spent climbing (s) | 10274 ± 4243 | 16 | 10999 ± 6146 | 15 | t | 0.7075 | 9703 ± 3265 | 16 | 9336 ± 5312 | 14 | t | 0.8251 | t | 0.6726 | t | 0.4417 |
| Time spent rearing (s) | 1475 ± 373 | 16 | 1465 ± 322 | 15 | t | 0.9360 | 1668 ± 470 | 16 | 1532 ± 465 | 14 | t | 0.4352 | t | 0.2093 | t | 0.6570 |
| Time spent grooming (s) | 2907 ± 989 | 16 | 2768 ± 845 | 15 | t | 0.6780 | 2766 ± 820 | 16 | 2670 ± 1089 | 14 | t | 0.7899 | t | 0.6641 | t | 0.7887 |
| Time immobile (s) | 13124 ± 3843 | 16 | 12283 ± 6087 | 15 | t | 0.6522 | 11369 ± 3865 | 16 | 12320 ± 4191 | 14 | t | 0.5257 | t | 0.2075 | t | 0.9849 |
| Exploration | ||||||||||||||||
| IC-Pheromone habituation: Time exploring (s) | 2531 ± 215 | 16 | 2518 ± 203 | 16 | t | 0.8636 | 2576 ± 186 | 16 | 2591 ± 236 | 14 | t | 0.8531 | t | 0.5272 | t | 0.3766 |
| Hole board: Visits (#) | 28.6 ± 9.1 | 16 | 29.8 ± 13.0 | 16 | t | 0.7553 | 25.6 ± 8.3 | 16 | 21.1 ± 9.3 | 14 | U | 0.1075 | t | 0.3393 | U | 0.0680 |
| Marble test (stereotypy) | ||||||||||||||||
| Buried marbles (#) | 13.6 ± 4.4 | 16 | 13.6 ± 6.4 | 16 | t | > 0.9999 | 12.1 ± 6.3 | 16 | 13.3 ± 7.8 | 14 | t | 0.6596 | t | 0.4401 | t | 0.8982 |
| Bar test (catalepsy) | ||||||||||||||||
| Time immobile on bar (s) | 0.1 ± 0.1 | 16 | 0.1 ± 0.0 | 14 | U | 0.3139 | 0.1 ± 0.1 | 16 | 0.1 ± 0.1 | 13 | U | > 0.9999 | U | 0.1579 | U | 0.0426 |
| Hurdle test (executive function) | ||||||||||||||||
| Crossings (#) | 8.0 ± 2.9 | 16 | 8.8 ± 2.5 | 16 | U | 0.2352 | 8.9 ± 4.3 | 16 | 9.3 ± 3.2 | 14 | U | 0.4114 | U | 0.7702 | t | 0.6564 |
| Escape latency (s) | 35.3 ± 12.3 | 16 | 43.5 ± 23.0 | 16 | U | 0.5641 | 54.7 ± 33.4 | 16 | 44.3 ± 25.8 | 14 | U | 0.2751 | U | 0.0796 | U | 0.9510 |
| Ratio (Latency/(Crossings+1)) | 4.1 ± 1.4 | 16 | 4.5 ± 2.3 | 16 | U | 0.7240 | 6.3 ± 4.5 | 16 | 4.4 ± 2.7 | 14 | U | 0.1661 | U | 0.1188 | U | 0.6971 |
| Startle | ||||||||||||||||
| Startle response at 120 db (AU) | 2.8 ± 2.0 | 16 | 2.3 ± 1.3 | 16 | U | 0.7450 | 2.0 ± 1.5 | 16 | 1.8 ± 0.8 | 14 | U | 0.5451 | U | 0.2203 | U | 0.3827 |
| IC Pheromone preference | ||||||||||||||||
| Habituation: Time on target site (s) | 1111 ± 257 | 16 | 1062 ± 141 | 16 | t | 0.5116 | 1120 ± 190 | 16 | 1236 ± 227 | 14 | t | 0.1430 | t | 0.9121 | t | 0.0215 |
| Test phase: Time on target site (s) | 1548 ± 382 | 16 | 1584 ± 288 | 16 | t | 0.7674 | 1675 ± 211 | 16 | 1682 ± 375 | 14 | t | 0.9489 | t | 0.2581 | t | 0.4337 |
| Habituation vs. Test | t-test | p < 0.0008 | t-test | p < 0.0001 | t-test | p < 0.0001 | t-test | p < 0.00010 | ||||||||
| IC Sucrose preference (anhedonia) | ||||||||||||||||
| Preference to sucrose bottle (%) | 99.4 ± 0.7 | 16 | 98.9 ± 1.1 | 16 | U | 0.3737 | 99.8 ± 0.3 | 16 | 99.6 ± 0.6 | 14 | U | 0.5929 | U | 0.0112 | U | 0.0519 |
| IC phenotyping (place & reversal learning) | ||||||||||||||||
| Place learning: Place error (%) | 69.5 ± 4.4 | 16 | 69.4 ± 4.6 | 16 | t | 0.9623 | 68.2 ± 5.3 | 16 | 71.8 ± 3.7 | 14 | t | 0.0345 | t | 0.4465 | t | 0.1241 |
| Reversal learning: Place error (%) | 66.8 ± 6.2 | 16 | 69.0 ± 4.6 | 16 | t | 0.2599 | 70.2 ± 5.8 | 16 | 71.7 ± 4.5 | 14 | U | 0.2572 | t | 0.1167 | t | 0.1204 |
| IC phenotyping (What-Where-When memory): Preference to (former) sucrose corner (%) | ||||||||||||||||
| Acquisition | 27.0 ± 7.8 | 16 | 30.5 ± 6.1 | 16 | t | 0.1605 | 24.1 ± 10.2 | 16 | 30.1 ± 7.3 | 14 | t | 0.0728 | t | 0.3790 | t | 0.8637 |
| Retrieval | 37.8 ± 9.2 | 16 | 40.2 ± 9.8 | 16 | t | 0.4805 | 34.5 ± 13.9 | 16 | 43.0 ± 8.4 | 14 | t | 0.0505 | t | 0.4345 | t | 0.4092 |
| day1 vs day2 | t-test | p < 0.0013 | t-test | p < 0.0026 | t-test | p < 0.0234 | t-test | p < 0.0002 | ||||||||
P values withstanding Bonferroni correction (α < 0.0125) are bolded.
IC intelliCage, t two-sided Welch’s corrected t-test, U two-sided Mann–Whitney U-test.