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. 2022 Feb 11;13:726153. doi: 10.3389/fimmu.2022.726153

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Copyright © 2022 Dölling, Eckstein, Singh, Schauer, Schoen, Shan, Bozec, Knopf, Schett, Muñoz and Herrmann

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutrophils with high nuclear levels of HIF-1α under hypoxia display viable morphologies. Serial slices of neutrophil infiltrates of ischemic areas of human acute myocardial infarction were stained by immunofluorescence for HIF-1α (green), neutrophil elastase (NE, green) and DNA (PI, red) (A). Overview (B, upper panel) and composite details (B, lower panels) of HIF-1α positive nuclei. The size bar represents 400 µm. Neutrophils with condensed nuclei (viable) showed bright HIF-1α staining (B, left lower panel), whereas neutrophils with decondensed nuclei (early stage of NET formation) contained less HIF-1α (B, right lower panel). The pattern and intensity of HIF-1α inversely correlated (Pearson r -0.5574, p <0.0001) with the nuclear area of neutrophils (C). Round and square dots distinguish the sample of AMI analyzed (two samples). MFI, mean fluorescence intensity.