Table 1.

Putative Transcription Regulatory Sequences in novel Nobecoviruses from Madagascar fruit bats.

Coronavirus accession number	ORF	TRS location (nt)	Leader TRS (nt)	TRS region	Intergenic TRS	Distance from TRS to AUG (nt)
OK067319	ORF1ab	65–70	UGAA	ACGAAC	UUAAU	22
	S	20,667–20,672	GUGA	ACGAAC	UUGUG	69
	NS3	24,593–24,598	AAAG	ACGAGC	UUAAUG	3
	E	25,240–25,245	UUUA	ACGAAC	GUCAUG	3
	M	25,451–25,456	UUGA	ACGAAC	AACAA	15
	N	26,170–26,175	UUGA	ACGAAA	UUAAA	6
	NS7a	27,651–27,656	UUGA	ACGAAG	AUG	0
	NS7b	28,441–28,446	GUUG	AAGAAC	UUUAA	7
OK067320	ORF1ab	55–60	UUGA	ACGAAC	UAAAA	14
	S	20,790–20,795	UUGA	ACGAAC	UUGUU	21
	NS3	24,575–24,580	GUAA	ACGAAC	UGUAUA	6
	E	25,293–25,298	GAUG	UCGAAC	UAUAAUG	4
	M	25,508–25,513	UUGA	ACGAAC	AACAA	18
	N	26,255–26,260	UUGA	ACGAAC	CAAUUAUG	5
	NS7a	27,674–27,679	UUGA	ACGAAC	AUG	0
	NS7b	28,046–28,051	UUUU	AUCAAC	CCGGG	28
	NS7c	28,256–28,261	UUGA	ACGAAC	CUAUG	2
OK067321	ORF1ab	119–124	UUGA	ACGAAC	UAAAA	14
	S	20,854–20,859	UUGA	ACGAAC	UUGUU	21
	NS3	24,666–24,671	GUAA	ACGAAC	UGUAUA	6
	E	25,384–25,389	GAUG	UCGAAC	UAUAATG	4
	M	25,599–25,604	UUGA	ACGAAC	AACAA	18
	N	26,346–26,351	UUGA	ACGAAC	CAAUUAUG	5
	NS7a	27,771–27,776	UUGA	ACGAAC	AUG	0
	NS7b	28,216–28,221	GUCG	AGAAAG	AGACC	15
	NS7c	28,353–28,358	UUGA	ACGAAC	AAAUG	2

Bold sections denote start codon AUG within 5 nucleotides of the beginning of the intergenic TRS region.