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. 2022 Feb 11;16:814867. doi: 10.3389/fncel.2022.814867

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Copyright © 2022 Xu, Chen, Fu, Yang, Yang, Huo, Tan, Chen and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interleukin-17A expression in mouse brains triggered by E. coli infection. Quantification of IL-17A expression in the brains of E. coli challenged mice at both the transcriptional and protein levels by applying QuantiGene Plex 2.0 Assay (A) or Procartaplex Multiplex Immunoassays (B). **p < 0.01 and ***p < 0.001 by one-way ANOVA analysis. Data were collected and presented as mean ± SD from three replicates at each time point. (C) Real-time PCR verification of the IL-17A mRNA transcription in the brains of E. coli challenged mice. Data were collected and presented as mean ± SD from three replicates at each time point. *p < 0.05 and ***p < 0.001 by one-way ANOVA analysis. (D) Western blot verification of the IL-17A protein expression in the brains of E. coli challenged mice. β-Actin was used as the loading control.