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. 2022 Feb 24;5:166. doi: 10.1038/s42003-022-03110-8

Fig. 1. Inhibition of the mycobacterial F1Fo-ATP synthase by amiloride and hexamethylene amiloride.

Fig. 1

a Structure of amiloride and b 5-(N,N-hexamethylene)amiloride (HMA). c ATP synthesis activity of NADH-energized M. smegmatis inverted membrane vesicles (IMV), treated with either 64 μM of amiloride, 64 μM of HMA, or 2 μM of BDQ as indicated. Error bars represent standard deviation from triplicate biologically independent measurements. d Time course of DCCD labelling in purified M. phlei c-rings in the presence of the indicated amounts of HMA. Data are representative of duplicate measurements.