Skip to main content
. 2022 Feb 24;13(2):179. doi: 10.1038/s41419-022-04630-1

Fig. 5. SF3B4 facilitates efficient splicing of RAD52.

Fig. 5

A Sashimi plot visualization of RNA-seq reads mapping to RAD52 in HEY cells in response to SF3B4 knockdown. B Schematic diagram showing two splicing variants of the RAD52 mRNA transcript identified from the Ensemble genome browser. C, D Relative mRNA expression of RAD52 splicing variants described in (B) from TCGA ovarian cancer database (202: n = 426; 206: n = 426; HGSOC: n = 426; Ovary: n = 88; FT: n = 5). E Schematic diagram showing the position of intron retention and primers that used for RT-PCR (n = 3 biologically independent samples). F, G Relative mRNA expression of RAD52 transcripts in ovarian cancer cells after SF3B4 knockdown (n = 3 biologically independent samples). H The interaction between SF3B4 and RAD52 mRNA was validated by RIP-PCR in HEY cells (n = 3 biologically independent samples). P-value was obtained by Unpaired t-test. *P < 0.05, **P < 0.01.