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. 2022 Feb 24;12:3143. doi: 10.1038/s41598-022-07236-x

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© The Author(s) 2022, corrected publication 2022

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Experimental schematic for labor induction with oxytocin in term pregnant rats. (A) A cartoon depicting the programming and implantation of iPRECIO pump in a pregnant rat followed by birth of healthy pups. (B) Experimental schematic showing the overall experimental outline with two separate cohorts for cesarean and vaginal delivery. In the vaginal delivery cohort, there were three sets of independent experiments for (i) intrauterine telemetry, (ii) RNA-seq experiments to assess the impact of in utero exposure to anesthesia and surgery on the cortical transcriptome of the newborn brain, and (iii) examination of oxidative stress in newborn pups after either Oxt or saline, respectively.