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. 2022 Feb 24;12:3184. doi: 10.1038/s41598-022-07156-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Amount of growth factors in dried CE and enhancement of keratinocyte proliferation by dried CE extracts. (a) Amount of growth factors and cytokines in CEs. Except for that in bFGF, the growth factor levels in the two types of CEs did not differ significantly (the amount of bFGF in dried CE was significantly higher than that in cryopreserved CE. * P < 0.05). The amount of IL-6 was less than the detection limit, and the amounts of IFN-γ and PDGF-BB were below the lower limit of quantification in both groups. (n = 5). (b) Absorbance correlated with keratinocyte proliferation in the WST-8 assay, which is exhibited on the vertical axis. The keratinocytes in the negative control group were cultured in keratinocyte culture medium (EpiLife) only, and a 1% human keratinocyte growth supplement was added to the positive control group. Keratinocyte proliferation was significantly higher on day 7 in the dried CE group than in the negative control group (n = 3, **P < 0.01). CE, cultured epidermis.