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. 2022 Feb 24;12:3184. doi: 10.1038/s41598-022-07156-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Wounds on the skin defect created on the back of diabetic mice. (a) Yellow lines indicate the wound area. In the cryopreserved CE and dried CE groups, no wounds remained on the representative samples on day 14. (b) The wound areas in the cryopreserved CE, dried CE, and control groups on days 4, 7, and 14 are depicted. Four and seven days after the operation, the wound areas in the two CE groups were significantly smaller than that in the control group (*P < 0.05, **P < 0.01, n = 6). The wound almost closed in all groups 14 days after the operation. CE, cultured epidermis.