Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 24;12:3184. doi: 10.1038/s41598-022-07156-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Microscopic images of wound areas in mice of control, cryopreserved CE, and dried CE groups. (a) The hematoxylin–eosin stained sections of the wounds in control, cryopreserved CE, and dried CE groups. The arrows indicate the newly formed epithelium. (b) Sections stained with anti-STEM121 in the wounds of control, cryopreserved CE, and dried CE groups. The red arrowheads (filled triangle) indicate cultured epidermis. The recipient murine keratinocytes can be easily distinguished from the transplanted CE. (c) Length of the epithelium in the wounds in control, cryopreserved CE, and dried CE groups. The epithelial lengths of the two CE groups were significantly higher than those of the control group on days 4 and 7, and significant difference was not observed between the two CE groups. Significant differences between the groups were not observed on day 14. (**P < 0.01, n = 6). (d) Transmission electron microscopy (TEM) images of the wound edge in control, cryopreserved CE, and dried CE groups. The wounds were harvested on day 2 and observed using TEM. The yellow arrowheads (filled triangle) indicate the boundary between the implanted CE and recipient keratinocytes. The transplanted human CE and the cell membrane of the mouse keratinocytes completely adhered without any gaps. CE, cryopreserved epidermis.