Fig. 3. Ubc9 deficiency intrinsically affects peripheral T-cell development.

Recipient CD45.1 (WT) mice (n = 5) were lethally irradiated (1000–1100 cGy, two split doses, 4 h apart) and each mouse was intravenously injected 1 × 10⁷ total mixed BM cells from CD45.1 (WT) and CD45.2 (KO) mice at the ratio of 1:1. The mice were sacrificed for further analysis eight weeks after the transplantation. A Occupation of WT and KO cells was determined in CD4 gate. B, C Apoptosis (WT: 7.39 ± 0.51% vs. KO: 6.03 ± 0.38%, p = 0.06) and proliferation (WT: 9.56 ± 1.44% vs. KO: 4.91 ± 0.89%, p < 0.05) of CD4 T cells from different sources were examined. D–F Detection of various T-cell subsets was conducted by flow-cytometry analysis. Th1: WT: 8.33 ± 0.89% vs. KO: 1.02 ± 0.09%, p < 0.01; Th2: WT: 4.49 ± 0.66% vs. KO: 0.14 ± 0.04%, p < 0.01; Th17: WT: 1.30 ± 0.19% vs. KO: 1.05 ± 0.18%, p = 0.39; Treg: WT: 11.53 ± 1.27% vs. KO: 4.78 ± 0.36%, p < 0.01. The p-value was determined by Student’s unpaired t-test.