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. 2022 Feb 24;13(2):178. doi: 10.1038/s41419-022-04635-w

Fig. 4. PCLAF can regulate PTTG1 to promote G1/S cell cycle transition.

Fig. 4

(A, B) RNA-seq analyzed the mRNA level of the cell cycle signaling pathway after PCLAF knockdown; (C) Western blotting examined the protein expression of PCLAF in 5 neuroblastoma cell lines; (D) The expression of PTTG1 and PCLAF in clinical tissues was measured by western blot; (E) Spearman correlation analysis of the relationship between PCLAF and PTTG1 in identical clinical tissues (r = 0.774, P < 0.01); (F, G) qRT-PCR and Western blot were used to detect the changes in PTTG1 mRNA and protein levels after interference PCLAF in SK-N-BE(2) and SH-SY5Y cell lines; (H, I) Interference efficiency of 3 small interfering RNAs with different sequences on PTTG1 in SK-N-BE (2) and SH-SY5Y cell lines, detected by qRT-PCR and Western blotting; (J, K) Flow cytometry was used to analyze the cell cycle of neuroblastoma cell lines. (*p < 0.05, **p < 0.01, ***p < 0.001and ****p < 0.0001, and bar graphs represent the mean ± SEM).