Biofilm formation in CR-hvKP strains upon treatment with VC
(A and B) Biofilm formation in KP1088 (A) and HvKP3 (B) upon treatment with different concentrations of VC for 24 h. Data are representative of their experiments performed in triplicate.
(C and D) Viable cell counting of KP1088 (C) and HvKP3 (D) biofilm treated with various concentrations of VC for 24 h.
(E) CLSM imaging of biofilm formation in strain KP1088 and HvKP3 upon treatment with VC. The left column is images of biofilm of KP1088 without treatment and after treatment with 8 mg/mL and 16 mg/mL of VC. The right column is images of HvKP3 biofilm without treatment and after treatment with 8 mg/mL and 16 mg/mL of VC.
(F) COMSTAT analysis of biovolumes from KP1088 biofilm treated with VC.
(G) COMSTAT analysis of biovolumes from HvKP3 biofilm treated with VC. ∗, p < 0.05, ∗∗, p < 0.005, ∗∗∗p < 0.0005, ∗∗∗∗p < 0.00005 (independent t-test).