Figure 6.

Inhibition of Nile Red efflux by VC in a dose-dependent manner

(A–D) Intensity of Nile Red fluorescence in strain KP1088 after treatment with different concentrations of VC; (B) Nile red fluorescence intensity of KP1088 after treatment of VC, followed by washout of VC after staining; (C) Nile red fluorescence of HvKP3 treated with different concentrations of VC; (D) Nile red fluorescence of HvKP3 upon treatment of VC and washout of VC after staining. 50 mM of Glucose was added to the assay at 2.8 min; the fluorescence was monitored for another 20 min. All experiments were performed in triplicate, and the mean ± SD is shown