Figure 3.

The overexpression of SIRT1 promotes increased liver injury and inflammasome activation in response to endotoxin. (A) Quantification of liver injury blood markers (ALT and AST) indicates increased liver injury in SIRT^oe mice 6 hours after LPS/GalN treatment. (B) Hematoxylin and eosin staining of liver sections from WT and SIRT^oe mice confirms more severe parenchymal damage in LPS/GalN-SIRT^oe mice. (C) Western blot analysis supported increased apoptosis in LPS/GalN-SIRT^oe mice compared with LPS/GalN-WT mice. Immunohistochemistry in paraffin-embedded liver sections using an (D) anti-F4/80 and (E) anti-CD11b antibody, followed by quantification show increased presence of macrophages in livers from SIRT^oe mice compared with WT mice 6 hours after LPS/GalN. n = 5–6 animals/treatment group were analyzed. Values are mean ± SEM. ∗P < .05, ∗∗P < .01 (WT vs SIRT^oe). Representative microscopical images are shown at (B) ×4 and (D, E) ×10.