Figure 5.

The overexpression of SIRT1 promotes increased liver injury and inflammasome activation in response to LPS. (A) Quantification of liver injury markers (ALT and AST) in serum samples indicates increased liver injury in SIRT^oe mice 14 hours after LPS treatment. (B) Quantitative polymerase chain reaction analysis and (C) Western blot analysis shows increased IL-1β  gene expression and cleavage, overall supporting the activation of the inflammasome in SIRT^oe livers 6 hours after LPS. (D) Caspase-3 activity was quantified showing mild impact of LPS in WT hepatocyte cell death that was not increased in SIRT^oe primary hepatocytes. n = 4–7 animals/treatment group were analyzed. Values are mean ± SEM. ∗P < .05, ∗∗P < .01 (WT vs SIRT^oe). Results from in vitro experiments are representative analysis of n = 3 replicates per time point.