Figure 6.
The overexpression of SIRT1 promotes proinflammatory phenotype in BMDMs associated with activation of mTOR signaling and decreased autophagy. (A) Increased IL-1β secretion detected by enzyme-linked immunosorbent assay on supernatants of BMDMs treated with 100 ng/mL LPS for 24 hours. (B) Immunocytochemistry on cultured BMDMs using an anti-p65 antibody (red) indicates attenuated and delayed translocation of p65 into the nucleus in SIRToe BMDMs after LPS. Experiments were done twice in triplicate. Values are mean ± SEM. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001 (WT vs SIRToe). Representative microscopical images are shown at ×20 magnification.