Table 3.

Depth of coverage by plasma NGS and detection of EGFR mutation and mutation allele frequency (AF %).

	Tissue EGFR test		Plasma NGS test
Case	EGFR Mutation	Method	Average consensus coverage (X)	EGFR Mutation (HGVSp)	AF (%)
1	Exon19del	Sanger	9496.7	p.E746_A750del	0.33
2	Exon19del	Roche PCR	4830.58	p.E746_A750del	9.44
3	Exon19del	Roche PCR	14854.77	p.E746_A750del	7.15
4	Exon19del	Sanger	11864.58	p.E746_S752delinsV	50.7
5	Exon19del	Roche PCR	6524.41	-	ND
6	p.E746_T751delinsA	Sanger	12254.13	p.E746_T751delinsA	80.3
7	p.L858R	Roche PCR	13842.24	p.L858R	1.51
8	Exon19del	Sanger	8068.15	-	ND
9	p.L858R	Sanger	14565.16	-	ND
	p.E709K			-	ND
10	p.L747_A750delinsP	Sanger	7420.09	p.L747_A750delinsP	0.98
11	p.L858R	Roche PCR	10417.81	p.L858R	0.86
	p.S768I			p.S768I	0.25
12	Exon19del unspecified	Roche PCR	5722.85	p.E746_A750del	0.73

ND, not detected.