FIGURE 2.

Evaluation of the cell-penetrating activity of CPP-BoNTA in mouse N2a cells with 2 h incubation. (A,B) Immunofluorescence (IF) analysis of the cell-penetrating activity of CPP-BoNTA. (A) Representative images. Scale bars, 20 μm. (B) Quantification of the mean fluorescence intensity of BoNTA positive cells. The data are shown as mean ± SD (n = 3). Statistical analysis is performed using Student’s t test. (C) TissueFAXS cytometry analysis of co-localization of BoNTA proteins and SV2 receptors in mouse N2a cells. Three biological replicates are performed and 1,000 cells are analyzed for each replicate.