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. 2022 Jan 19;121(4):515–524. doi: 10.1016/j.bpj.2022.01.013

Figure 2.

Figure 2

Collagen mutants have a softer cuticle. (a) Young wild-type and dpy-13 worms' immediate response to hyperosmotic shock (0.35 to 1 OSM). The appearance of wrinkles in the young collagen gene mutant indicates a lower cuticular resistance. Scale bars, 100 μm. (b and c) Worms were transferred to 1 OSM solution and imaged after 20 min. Length and area changes of wild-type and dpy-13 worms at day 1 were calculated; N2/day 1: n = 12, dpy-13/day 1: n = 16. (d and e) Elastic moduli in radial and longitudinal directions for young wild-type and collagen mutants. N2/day 1: n = 10, dpy-13/day 1: n = 10. (f) Bulk moduli of wild-type and collagen mutants under small and large stretching loads. The cuticle defective mutant is significantly softer than wild-type. N2/day 1: n = 10, dpy-13/day 1: n = 10; two biological replicates. Two-tailed t-tests. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Plots show 25th percentile, median, and 75th percentile. To see this figure in color, go online.