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. 2022 Jan 19;121(4):515–524. doi: 10.1016/j.bpj.2022.01.013

Figure 5.

Figure 5

Stiffness changes with age are slowed in the daf-2 longevity mutant. (a) Aged wild-type and daf-2 worms' immediate response to hyperosmotic shock (0.35 to 1 OSM). Absence of wrinkles in the aged long-lived mutant signals better cuticle resistance. Scale bars, 100 μm. (b and c) Worms were transferred to 1 OSM solution and imaged after 20 min. Length and area changes of wild-type and daf-2 worms at day 1 and day 10 were calculated. Decreasing resistance with age is slowed in daf-2 mutant; N2/day 1: n = 12, N2/day 10: n = 8, daf-2/day 1: n = 12, daf-2/day 10: n = 8. (d and e) Elastic moduli in radial and longitudinal directions for young and aged wild-type and long-lived mutants. Increasing stiffness of cuticle with age is slowed down in daf-2 mutant. N2/day 1: n = 10, N2/day 10: n = 10, daf-2/day 1: n = 10, daf-2/day 10: n = 10. (f) Adulthood collagen gene knockdown reduced daf-2 ability to resist volumetric shrinkage upon transferring to 1 OSM solution. n = 8 for N2 and daf-2;(control). n = 14 for daf-2;(col-120 RNAi). Two biological replicates. One-way ANOVA with Dunnett's post-hoc. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Plots show 25th percentile, median, and 75th percentile. To see this figure in color, go online.