FIGURE 6.

Levels of proinflammatory cytokines and chemokines (C/C) are modulated by BDWG. Full‐thickness 6‐mm punch biopsy wounds were made on the backs of mice (4/group) and the wounds were UT, PT, or BT as described in Figure 2. At 1, 3, and 7 dPIT, tissue from the three biopsy wounds on a single mouse was collected using an 8‐mm punch and the tissues were pooled and treated as a single sample (four separate pools/group). An equal amount of tissue was collected from three uninjured mice to serve as baseline for levels of C/C within normal skin. Proteins were extracted from the tissues and concentration of protein in each sample was measured by the Bradford assay. Quantities of the C/C were measured using the U‐PLEX biomarker assay with MSD DISCOVERY WORKBENCH software version 4.0; data are reported in pg/mg of protein. Bars represent the means of the fold‐change values obtained from the four pools compared with the average value of the uninjured pool (Table S3); striped bars, UT; dotted bars, PT; solid bars, BT. A, Fold changes in levels of IL‐6, CXCL1, CCL3, IL‐1β, GM‐CSF, VEGF‐A, and CXCL10 at 1 dPIT; B, Levels of the C/Cs at 3 dPIT; C, Levels of the C/Cs at 7 dPIT. Differences in changes in expression were analysed using one‐way ANOVA with Tukey's multiple comparisons post‐test; *P < .05; **P < .001. BT, BDWG‐treated; PT, PEG‐treated; PEG, polyethylene glycol; UT, untreated