Table 1. PCR Primer sequences.
Where no probe sequence is shown, SYBR green dye was used. For pre-made primers, assay ID is provided. Forward (F), reverse (R), probe (P). Primer sets for Clcf1, Gbp2, Cst7, Myl9, S1pr3, Serping1 and Tgm1 were predesigned TAQMAN gene expression assays.
| Gene | Access. num. | BP | Sequence |
|---|---|---|---|
| C1qa | NM_007572.2 | 268 | F: 5’GCCGAGCACCCAACGGGAAGG3’ R: 5’GGCCGGGGCTGGTCCCTGATA3’ |
| C3 | NM_009778.2 | 115 | F: 5’AAAGCCCAACACCAGCTACA3’ R: 5’GAATGCCCCAAGTTCTTCGC3’ |
| Ctss | NM_021281 | 80 | F: 5’GCCACTAAAGGGCCTGTCTCT3’ R: 5’TCGTCATAGACACCGCTTTTGT3’ |
| Ccl2 | NM_011333 | 81 | F: 5’GTTGGCTCAGCCAGATGCA3’ R: 5’AGCCTACTCATTGGGATCATCTTG3’ P: 5’TAACGCCCCACTCACCTGCTGCTACT3’ |
| CD14 | NM_009841.4 | 128 | F: 5’GCCAAATTGGTCGAACAAGC3’ R: 5’CCATGGTCGGTAGATTCTGAAAGT3’ |
| Cxcl1 | NM_008176 | 82 | F: 5’CACCCAAACCGAAGTCATAGC3’ R:5’AATTTTCTGAACCAAGGGAGCTT3’ |
| Cxcl10 | M33266.1 | 127 | F: 5’GCCGTCATTTTCTGCCTCAT3’ R: 5’GCTTCCCTATGGCCCTCATT3’ P: 5’TCTCGCAAGGACGGTCCGCTG3’ |
| Gbp2 | NM_010260.1 | 94 | F: 5’CAGCTGCACTATGTGACGGA3’ R: 5’AGCCCACAAAGTTAGCGGAA3’ |
| Gfap | K01347.1 | 90 | F: 5’CTCCAACCTCCAGATCCGAG3’ R: 5’TCCACAGTCTTTACCAGATGT3’ |
| Gper1 | NM_029771.3 | 115 | F: 5’TGCTGCCATCCAGATTCAAG3’ R: 5’GGGAACGTAGGCTATGGAAAGAA3’ |
| Il1b | M15131 | 69 | F: 5’GCACACCCACCCTGCA3’ R: 5’ACCGCTTTTCCATCTTCTTCTT3’ P: 5’TGGAGAGTCTGGATCCCAAGCAATACCC3’ |
| Il1r1 | NM_001123382.1 | 117 | F: 5’GCAATATCCGGTCACACGAGTA3’ R: 5’ATCATTGATCCTGGGTCAGCTT3’ P: 5’TCCTGAGCCCTCGGAATGAGACGATC3’ |
| Il1a | NM_010554 | 150 | F: 5’TGTTGCTGAAGGAGTTGCCAG3’ R: 5’CCCGACTTTGTTCTTTGGTGG3’ |
| Nlpr3 | AY355340.1 | 115 | F: 5’GAGCCTACAGTTGGGTGAAATGT3’ R: 5’CCACGCCTACCAGGAAATCTC3’ |
| Ptx3 | X83601 | 62 | F: 5’ACAACGAAATAGACAATGGACTTCAT3’ R: 5’CTGGCGGCAGTCGCA3’ P: 5’CCACCGAGGACCCCACGCC3’ |
| Ccl5 | NM_013653 | 75 | F: 5’GCAGTCGTGTTTGTCACTCGAA3’ R: 5’GATGTATTCTTGAACCCACTTCTTCTC3’ |
| S100a10 | NM_009112.2 | 71 | F: 5’CTAGCCTCATCGTGGTGTGC3’ R: 5’GAGTCTGTCGAAACCTGGGC3’ |
| Steap4 | NM_054098.3 | 93 | F: 5’TGCAAGCCGGCAGGTGTTTGT3’ R: 5’TCCAGTGGGGTGAGCCCAAGA3’ |
| Tnf | M11731 | 149 | F: 5’CTCCAGGCGGTGCCTATG3’ R: 5’GGGCCATAGAACTGATGAGAGG3’ P: 5’TCAGCCTCTTCTCATTCCTGCTTGTGG3’ |
| Vim | NM_011701.4 | 100 | F: 5’GCTGCAGGCCCAGATTCA3’ R: 5’TTCATACTGCTGGCGCACAT3’ |