Figure 5.

Simultaneous single-camera-based measurements of V_m and Ca signals in isolated cell culture monolayers of neonatal rats. (A) Time-snapshots of V_m - Ca dynamic at different time points illustrating the presence of anchored spiral wave and spatial loss of correlation between propagating V_m wavefront from Ca dynamics. (B) Normalized V_m and Ca signals obtained from processed raw recordings showing Ca signal lagging in time. (C) Representative unfiltered V_m and Ca signal traces with no filtering expressed as a relative change. In cell culture monolayers, Ca signals have significantly higher SNR than V_m signals originating only from the dye bound to the cell membrane. The amount of cross-talk and the presence of V_m signal in Ca signal with excitation at near the semasbestic point (Exc550) results is a small yet negligible cross-talk compared to the amplitudes of the signals.