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. 2022 Feb 11;13:803445. doi: 10.3389/fphys.2022.803445

FIGURE 2.

FIGURE 2

MiR-122-5p inhibition attenuates PA-induced inflammatory and oxidative stress response in L02 cells. (A) L02 cells were transfected with lentivirus encoding miR-122-5p antisense (miR-122-5p inhibitor) or control sense [negative control (NC) inhibitor] for 48 h, and reverse transcription and quantitative PCR (RT-qPCR) analyzed the expression level of miR-122-5p. (B) Representative Oil Red O staining. (C) RT-qPCR analyzed the expression of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, and IL-8. (D) ELISA analyzed the level of TNF-α, IL-6, and IL-8 in culture supernatants. (E) The activity of SOD and GSH-Px, and the content of MDA. (F) Western blot analysis of PI3K, AKT, p-AKT, mTOR, and p-mTOR expression. Error bars indicate SD. *P < 0.05, **P < 0.01, ***P < 0.001.