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. 2022 Feb 11;13:803445. doi: 10.3389/fphys.2022.803445

FIGURE 4.

FIGURE 4

MiR-122-5p directly inhibits FOXO3 expression in L02 cells and hepatic tissues in obese mice. (A) Matched sequence (red box) of with mature miR-122-5p and the FOXO3 WT. (B) Targeted binding of miR-22-5p and FOXO3 were detected by fluorescein reporter. (C) qRT-PCR analysis of miR-122-5p and FOXO3 expression in L02 cells after transfection with miR-122-5p mimic. (D) Western blot analysis of FOXO3 expression after transfection with miR-122-5p mimic. (E) Western blot analysis of FOXO3 expression in L02 cells treated with different concentrations of PA. (F) Western blot analysis of FOXO3 expression in L02 cells treated with miR-122-5p inhibitor. (G) qRT-PCR analysis of hepatic FOXO3 expression after injected with miR-122-5p inhibitor. (H) Western blot analysis of hepatic FOXO3 expression after injected with miR-122-5p inhibitor. Error bars indicate SD. *P < 0.05, **P < 0.01, ***P < 0.001.