FIGURE 4.

Comparison of the role of FASN and lipases in the activity of orlistat. Obese monocytes were differentiated in the presence of Orlistat, C75 (50 μM) or Cay10499 (10 μM) before maturation with the MPLA cocktail. (A‐D) Mature FastDC were evaluated for expression of costimulatory molecules by flow cytometry (A) and their supernatants characterised by ELISA for IL‐10 (B) and IL‐12p70 content (C), from which the IL‐12p70 to IL‐10 ratio was calculated (D). (E‐G) PBL were co‐cultured with the different MPLA FastDC and evaluated after 18 h for the percentages of cells that secrete IFN‐γ (E) or degranulate in response to K562 (F) or RCC53 cells (G). (H) FastDC were also evaluated for the uptake of Bodipy500 and 2‐NBDG as well as stained with NileRed. Dotted lines highlight the brake of the y‐axis. Shown are the mean ± SE from five different donors. For the ELISA data, cytokine levels upon inhibitor treatment were normalised to untreated MPLA and are shown for each individual donor as x‐fold over MPLA FastDC (set to 1, dashed line). A‐H: *, p < 0.05 in ANOVA test