Figure 3.
Structure of Dicer and Dicer-like proteins determined by cryo-EM. (a) Functional domains of human Dicer protein. (b) Structure of human Dicer in complex with the TRBP protein, a component of the RISC complex (magenta surface) and the pre-let-7 [51]. (c) Structure of the Dicer-like protein 1 (DCL1) from A. thaliana in complex with the pre-miR-166f [71]. (d) Structure of the Dicer-like protein 3 (DCL3) from A. thaliana in complex with a synthetic 40 mer dsRNA [72]. All the structures were rendered and represented with Protein Imager software [39].