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. 2022 Jan 24;14(2):218. doi: 10.3390/v14020218

Figure 2.

Figure 2

Poly(I:C)-stimulated NPC are potent suppressors of HCV replication in vitro. Primary (a) KC, (b) LSEC and (c) HSC were stimulated with TLR1–9 agonists for 24 h and cell culture supernatants were collected (n = 4). Supernatants were co-cultured with the subgenomic HCV replicon system con1. After 72 h, RNA was extracted and HCV replication was determined by qRT-PCR. Cultured (d) KC, (e) LSEC and (f) HSC were stimulated with TLR1–9 agonists for 90 min, followed by extraction of total protein. Western blot analysis was performed with antibodies detecting GAPDH as well as phosphorylated and total IRF3. Original blots are given in the Supplementary Materials. (g,j) KC, (h,k) LSEC and (i,l) HSC were stimulated with TLR1–9 ligands for 6 h and RNA was extracted. Gene expression of MX1 and ISG15 was determined by qRT-PCR. Data represent copy numbers as mean ± SD normalized to 100,000 copies of reference gene ACTB. Asterisks indicate significant results (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001, **** p-value < 0.0001).