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. 2022 Jan 24;14(2):218. doi: 10.3390/v14020218

Figure 4.

Figure 4

Antiviral effect of NPC is mediated by the type I interferon receptor. Cell culture supernatants from poly(I:C)-treated (a) KCs, (b) LSECs and (c) HSCs (n = 3) were preincubated with neutralizing antibodies against IFNA, IFNB1 or IFNL for 3 h. In parallel, con1 cells were preincubated with neutralizing antibodies against interferon receptors for 3 h. After incubation, con1 cells were exposed to preincubated, antibody-treated or untreated supernatants and cultured for 72 h. (d) Neutralizing antibodies were preincubated with respective interferons for 3 h and co-cultured with con1 cells for 72 h. Afterwards, RNA was extracted and HCV replication was determined by qRT-PCR (normalized to ACTB, mean ± SD). Asterisks indicate significant results (** p < 0.01; *** p < 0.001; **** p-value < 0.0001). Abbreviations: Ø no supernatants, w/o supernatants without treatment.