Table 1.
Cytotoxicities of diphyllin 1 and cleistanthin B 8 for multiple cell types after a 48 h incubation.
| Cell Type | Diphyllin 1 | Cleistanthin B 8 | ||
|---|---|---|---|---|
| CC50 (µM) 1,2 | 95% CI (µM) | CC50 (µM) 1,2 | 95% CI (µM) | |
| Vero (48 h) | >100 | - | >100 | - |
| Vero (72 h) | >100 | - | >100 | - |
| Vero (144 h) | 18.19 | 15.1 to 21.65 | 80.73 | 66.54 to 104.2 |
| PS (48 h) | 92.44 | 62.9 to 140.5 | 78.86 | 48.17 to 129.40 |
| UKF-NB-4 (48 h) | 11.13 | 7.21 to 17.35 | 6.17 | 4.53 to 8.43 |
| Huh-7 (48 h) | 1.89 | 1.53 to 2.32 | 3.96 | 3.01 to 5.243 |
| HBCA (48 h) | 3.39 | 2.07 to 5.71 | 2.08 | 1.77 to 2.45 |
| BHK-21 (72 h) | >100 | - | - | - |
1 Determined from three biological replicates of two independent experiments.2 Expressed as a 50% reduction in cell viability and calculated from the inflection points of sigmoidal percentage cell viability curves, which were obtained by a nonlinear fit of transformed inhibitor concentrations versus normalized response using GraphPad Prism 7.04 (GraphPad Software, Inc., USA).