Table 2.
Sample | Liver | Kidney | Heart | Brain | Stomach | Spleen | Lung | Uterus | Urinary Bladder | Small Intestine | Cecum/Large Intestine | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HE | IHC | HE | IHC | HE | IHC | HE | IHC | HE | IHC | HE | IHC | HE | IHC | HE | IHC | HE | IHC | HE | IHC | HE | IHC | |
Control | - | - | - | - | - | - | - | - | - | - | - | - | - | - | NT | NT | NT | NT | NT | NT | NT | NT |
Control | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | NT | NT | NT | NT | NT | NT |
Control | - | - | - | - | NT | NT | - | - | - | - | NT | NT | - | - | NT | NT | NT | NT | - | - | - | - |
i.v. | + | - | ++ | + | + | + | - | - | - | - | - | - | - | - | NT | NT | - | + | NT | NT | NT | NT |
i.v. | - | - | ++ | + | + | + | - | - | - | - | - | + | Au | - | - | - | - | - | NT | NT | NT | NT |
i.v. | - | - | NT | NT | + | ++ | - | - | - | - | - | - | Au | - | NT | NT | NT | NT | - | - | - | + |
i.v + 1A8 | + | + | + | ++ | +++ | +++ | - | + | - | - | - | + | - | + | - | - | - | - | NT | NT | NT | NT |
i.v + 1A8 | + | + | +++ | +++ | +++ | +++ | - | - | - | + | - | + | - | - | NT | NT | - | - | NT | NT | NT | NT |
i.v + 1A8 | + | + | +++ | +++ | +++ | +++ | + | ++ | - | - | - | + | - | + | - | - | - | - | - | - | - | + |
Gavage | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | NT | NT | NT | NT |
Gavage | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | NT | NT | NT | NT |
Gavage | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + |
Gavage + 1A8 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | NT | NT | NT | NT | NT | NT |
Gavage + 1A8 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | NT | NT | NT | NT |
Gavage + 1A8 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + |
Lesion scoring (HE): - WNL; + minimal to mild inflammation, infiltration of neutrophils, and rare macrophages with rare microabscessation; ++ mild to moderate inflammation, infiltration of neutrophils and macrophages, apoptosis or inflammatory and parenchymal cells, and microabscessation, often with a necrotic core; +++ severe multifocal necrotizing inflammation, infiltration of neutrophils and macrophages with necrotizing microabscessation, and abscessation; ++++ severe multifocal to coalescing inflammation, infiltration of neutrophils and macrophages with necrotizing microabscessation, and abscessation. IHC: - negative; + 0-5 cells or yeasts per HPF (50x), macrophages, and rare yeast; ++ greater than 5 cell/HPF, intralesional in abscesses, yeast, and scattered antigen in the debris and intracytoplasmic in macrophages; +++ numerous cells/HPF, intralesional in abscesses, numerous yeasts, and scattered antigen in the debris and intracytoplasmic in macrophages; ++++ numerous cells/HPF, intralesional in abscesses, copious amounts of yeast, and scattered antigen in the debris and intracytoplasmic in macrophages. NT: Tissue not available for evaluation. Au: Marked autolysis.