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. 2022 Feb 1;10(2):330. doi: 10.3390/microorganisms10020330

Table 2.

Fungal organ burden scoring system for neutrophil microbiota-depleted mice infected with C. auris. A comparison of the routes of infection and neutrophil depletion shows the following: Lesion scoring (HE): - within normal limits (WNL); + minimal to mild suppurative inflammation, scattered macrophages, and rare microabscessation; ++ mild to moderate suppurative inflammation, moderate numbers of macrophages, and micro abscesses; +++ severe multifocal necrotizing suppurative inflammation, numerous macrophages, and microabscesses; ++++ severe multifocal to coalescing necrotizing suppurative inflammation, numerous macrophages, and microabscesses.

Sample Liver Kidney Heart Brain Stomach Spleen Lung Uterus Urinary Bladder Small Intestine Cecum/Large Intestine
HE IHC HE IHC HE IHC HE IHC HE IHC HE IHC HE IHC HE IHC HE IHC HE IHC HE IHC
Control - - - - - - - - - - - - - - NT NT NT NT NT NT NT NT
Control - - - - - - - - - - - - - - - - NT NT NT NT NT NT
Control - - - - NT NT - - - - NT NT - - NT NT NT NT - - - -
i.v. + - ++ + + + - - - - - - - - NT NT - + NT NT NT NT
i.v. - - ++ + + + - - - - - + Au - - - - - NT NT NT NT
i.v. - - NT NT + ++ - - - - - - Au - NT NT NT NT - - - +
i.v + 1A8 + + + ++ +++ +++ - + - - - + - + - - - - NT NT NT NT
i.v + 1A8 + + +++ +++ +++ +++ - - - + - + - - NT NT - - NT NT NT NT
i.v + 1A8 + + +++ +++ +++ +++ + ++ - - - + - + - - - - - - - +
Gavage - - - - - - - - - - - - - - - - - - NT NT NT NT
Gavage - - - - - - - - - - - - - - - - - - NT NT NT NT
Gavage - - - - - - - - - - - - - - - - - - - + - +
Gavage + 1A8 - - - - - - - - - - - - - - - - NT NT NT NT NT NT
Gavage + 1A8 - - - - - - - - - - - - - - - - - - NT NT NT NT
Gavage + 1A8 - - - - - - - - - - - - - - - - - - - + - +

Lesion scoring (HE): - WNL; + minimal to mild inflammation, infiltration of neutrophils, and rare macrophages with rare microabscessation; ++ mild to moderate inflammation, infiltration of neutrophils and macrophages, apoptosis or inflammatory and parenchymal cells, and microabscessation, often with a necrotic core; +++ severe multifocal necrotizing inflammation, infiltration of neutrophils and macrophages with necrotizing microabscessation, and abscessation; ++++ severe multifocal to coalescing inflammation, infiltration of neutrophils and macrophages with necrotizing microabscessation, and abscessation. IHC: - negative; + 0-5 cells or yeasts per HPF (50x), macrophages, and rare yeast; ++ greater than 5 cell/HPF, intralesional in abscesses, yeast, and scattered antigen in the debris and intracytoplasmic in macrophages; +++ numerous cells/HPF, intralesional in abscesses, numerous yeasts, and scattered antigen in the debris and intracytoplasmic in macrophages; ++++ numerous cells/HPF, intralesional in abscesses, copious amounts of yeast, and scattered antigen in the debris and intracytoplasmic in macrophages. NT: Tissue not available for evaluation. Au: Marked autolysis.