Figure 5.

The influence of CA on splenic gene expression in PD mice based on RNA-Seq. (a) Volcano plot of splenic DEGs between the MPTP and CA+MPTP groups. (b) Volcano plot of colonic DEGs between the MPTP and CA+MPTP groups. The heatmaps showing the results of cluster analysis for DGEs between the control, MPTP and CA+MPTP groups in the spleen (c) and colon (d). CA pretreatment significantly restored the gene expression in the colon and spleen of MPTP mice; combining with the regulation of CA on IL-17, IFN-γ and TGF-β in PD mice, it was indicated that the neuroprotective effects of CA were closely related to the modulation of CA on peripheral immune system. Cut-off value of (|log₂FC|) > 1.2 and padj < 0.05, n = 3 per group. S: spleen, C: colon. CAM: CA+MPTP group, M: MPTP group, NC: control group.