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. 2022 Feb 17;9(2):62. doi: 10.3390/jcdd9020062

Figure 2.

Figure 2

Spatio-temporal expression of DCHS1-LIX1L-SEPT9 proteins throughout development. IHC staining of (A) DCHS1, (B) LIX1L and (C) SEPT9 (red), MF20 (green), and nuclei (Hoechst, blue) on sister sections of E15.5, P0 and four-month-old wildtype mouse valve tissue reveals similar expression throughout the interstitium with localization to the leaflet tips by P0 (arrows) and restriction to the endothelium through adulthood. AL = anterior leaflet, PL = posterior leaflet.